Almost all of the functions in a cell or organism can trace their beginnings to the cell nucleus with the turn on of a gene, processing of its RNA, and subsequent transport of that RNA into the cytoplasm where it is synthesized into a protein. Yet very little is known about how these events are spatially and temporally coordinated within the context of the nucleus to allow for the regulation of accurate gene expression; or how they go awry resulting in various diseases.
Our laboratory has focused on implementing innovative approaches to image gene expression within the context of the living cell. Such approaches allow us to gain important insight into the spatial and temporal aspects of gene expression that are difficult, if not impossible, to acquire using in vitro approaches. To complement our cell biological studies we have taken a molecular approach to identify and characterize large nuclear retained non-coding RNAs that may have roles in regulating various aspects of gene expression and/or nuclear organization. This interdisciplinary approach will establish the in vivo parameters necessary for efficient gene expression and will serve as the basis from which to study alterations in nuclear function related to various diseases.
David L. Spector: Phone (516) 367-8456; email email@example.com
Laboratory Phone: (516) 367-8478
Laboratory Fax: (516) 367-8876
One Bungtown Road
Cold Spring Harbor, NY 11724