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Live cell observations of entry of the pre-mRNA splicing factor SF2/ASF into daughter cell nuclei
HeLa cells were transiently transfected with YFP-SF2/ASF and live-cell observations were initiated 2 days
post-transfection. Metaphase cells exhibit 1-2 mitotic interchromatin granule clusters
(MIGs) near the metaphase plate. As the cell enters anaphase, MIGs become more abundant.
SF2/ASF begins to enter daughter nuclei at telophase. Nuclear entry of
YFP-SF2/ASF is nearly complete in approximately 20 min.
From: Prasanth, K.V., Sacco-Bubulya, P.A., Prasanth, S.G. and D. L.
Spector. 2003. Sequential entry of components of the gene expression machinery into daughter nuclei.
Molec. Biol. Cell. 14, 1043-1057.
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