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Selected Publications

Spector, D.L. 1990. Higher order nuclear organization: 3-dimensional distribution of small nuclear ribonucleoprotein particles. Proc. Natl. Acad. Sci. USA 87: 147-151. 

Potashkin, J.A., Derby, R.J., and Spector, D.L. 1990. Differential distribution of factors involved in pre-mRNA processing in the yeast cell nucleus. Mol. Cell. Biol. 10: 3524-3534. 

Spector, D.L., Fu, X.-D., and Maniatis, T. 1991. Associations between distinct pre-mRNA splicing components and the cell nucleus. EMBO J. 10: 3467-3481.

Huang, S. and Spector, D.L. 1991. Nascent pre-mRNA transcripts are associated with nuclear regions enriched in splicing factors. Genes & Dev., 5: 2288-2302.

Huang, S. and Spector, D.L. 1992. U1 and U2 snRNAs are present in nuclear speckles. Proc. Natl. Acad. Sci. USA 89: 305-308.

O'Keefe, R.T., Henderson, S.C. and Spector, D.L. 1992. Dynamic organization of DNA replication in mammalian cell nuclei; spatially- and temporally-defined replication of chromosome specific a-satellite DNA sequences. J. Cell Biol., 116: 1095-1110.

Huang, S. and Spector, D.L. 1992. Will the real splicing sites please light up! Current Biology, 2: 188-190.

Spector, D.L., Lark, G. and Huang, S. 1992. Differences in snRNP localization between transformed and non-transformed cells. Mol. Biol. Cell, 3: 555-569.

Jiménez-García, L.F. and Spector, D.L. 1993. In vivo evidence that transcription and splicing are coordinated by a recruiting mechanism. Cell 73: 47-59.

Spector, D.L. 1993. Nuclear organization of pre-mRNA processing. Current Opinion in Cell Biology 5: 442-447.

Jiménez-García, L.F., Green, S.R., Mathews, M.B. and Spector, D.L. 1993. Organization of the double-stranded RNA-activated protein kinase DAI and virus-associated VA RNA1 in adenovirus-2 infected HeLa cells. J. Cell Sci. 106: 11-22.

Spector, D.L. 1993. Macromolecular domains within the cell nucleus. Annu. Rev. Cell Biol. 9: 265-315.

Spector, D.L., O'Keefe, R.T., and Jiménez-García, L.F. 1993. Compartmentalization of transcription and pre-mRNA splicing within the mammalian cell nucleus. Cold Spring Harbor Symp. Quant. Biol. 58: 799-805.

O'Keefe, R.T., Mayeda, A., Sadowski, C.L., Krainer, A.R. and Spector, D.L. 1994. Disruption of pre-mRNA splicing in vivo results in reorganization of splicing factors. J. Cell Biol. 124: 249-260.

Huang, S., Deerinck, T.J., Ellisman, M.H. and Spector, D.L. 1994. In vivo analysis of the stability and transport of nuclear poly(A)+ RNA. J. Cell Biol. 126: 877-899.

Deerinck, T.J., Martone, M.E., Lev-Ram, V., Green, D.P.L., Tsien, R.Y., Spector, D.L., Huang, S. and Ellisman, M.H. 1994. Fluorescence photooxidation with eosin: a method for high resolution immunolocalization and in situ hybridization detection for light and electron microscopy. J. Cell Biol. 126: 901-910.

Jiménez-García, L.F., Segura-Valdez, M de L., Ochs, R.L. and Spector, D.L. 1994. Nucleologenesis: U3 snRNA-containing prenucleolar bodies move to sites of active pre-rRNA transcription after mitosis. Molec. Biol Cell. 5: 955-966.

Tani, T., Derby, R.J., Hiraoka, Y and Spector, D.L. 1995. Nucleolar accumulation of poly(A)+ RNA in heat-shocked yeast cells: Implication of nucleolar involvement in mRNA transport. Molec. Biol. Cell 6: 1515-1534.

Huang, S. and Spector, D.L. 1996. Intron-dependent recruitment of pre-mRNA splicing factors to sites of transcription. J. Cell Biol. 133: 719-732.

Huang, S. and Spector, D.L. 1996. The dynamic organization of pre-mRNA splicing factors. J. Cellular Biochem. 62: 191-197.

Misteli, T. and Spector, D.L. 1996. Ser/Thr phosphatase 1 modulates the subnuclear distribution of pre-mRNA splicing factors. Molec. Biol. Cell 7: 1559-1572.

Spector, D.L. 1996. Nuclear organization and gene expression. Exp. Cell Res. 229: 189-197.

Misteli, T. and D.L. Spector. 1997. Protein phosphorylation and the nuclear organization of pre-mRNA splicing. Trends in Cell Biol. 7: 135-138.

Huang, S., T.J. Deerinck, M.H. Ellisman and D.L. Spector. 1997. The dynamic organization of the perinucleolar compartment in the cell nucleus. J. Cell Biol. 137: 965-974.

Misteli, T., J.F. Cáceres and D.L. Spector. 1997. The dynamics of a pre-mRNA splicing factor in living cells. Nature 387: 523-527.

Huang, S., T.J. Deerinck, M.H. Ellisman and D.L. Spector. 1997. The dynamic organization of the perinucleolar compartment in the cell nucleus. J. Cell Biol., 137: 965-974.

Powell, R.D., C.M.R. Halsey, D.L. Spector, S.L. Kaurin, J. McCann and J.F. Hainfeld. 1997. A covalent fluorescent-gold immunoprobe: Simultaneous detection of a pre-mRNA splicing factor by light and electron microscopy. J. Histochem. Cytochem., 45: 947-956.

Huang, S. and Spector, D.L. 1997. Pre-mRNA splicing: Nuclear organization of factors and substrates. In: Eukaryotic Messenger RNA Processing: Frontiers in Molecular Biology. (A.R. Krainer, Ed), pp. 37-67.

Huang, S. , A. Mayeda, A.R. Krainer and D.L. Spector. 1997. RCC1 and nuclear organization. Molec. Biol. Cell., 8: 1143-1157.

Misteli. T. and D.L. Spector. 1997. Applications of the green fluorescent protein in cell biology and biotechnology. Nature Biotechnology 15, 961-964.

Spector, D.L. Genes and transcripts in the interphase nucleus. In: Nuclear Organization, Chromatin Structure and Gene Expression, van Driel, R. and Otte, A.P., eds. Oxford University Press, pp. 169-184.

Cáceres, J.F., T. Misteli, G.R. Screaton, D.L. Spector and A.R. Krainer. 1997. Role of the modular domains of SR proteins in subnuclear localization and alternative splicing specificity. J. Cell Biol. 138: 225-238.

Lorenz, P., B.F. Baker, C.F. Bennett and D.L. Spector. 1998. Phosphorothioate antisense oligonucleotides induce the formation of nuclear bodies. Molec. Biol. Cell., 9, 1007-1023.

Misteli, T. and D.L. Spector. 1998. The cellular organization of gene expression. Curr. Opin. Cell Biol. 10, 323-331.

Misteli, T., J.F. Cáceres, J.Q. Clement, A.R. Krainer, M.F. Wilkinson, and D.L. Spector. 1998. Serine phosphorylation of SR proteins is required for their recruitment to sites of transcription in vivo. J. Cell Biol. 143, 297-307.

Misteli, T. and D.L. Spector. 1999. RNA polymerase II targets pre-mRNA splicing factors to transcription sites in vivo. Molecular Cell 3, 697-705.

Tvaruskó, W., M. Bentele, T. Misteli, R. Rudolf, C. Kaether, D.L. Spector, H.H. Gerdes, and R. Eils. 1999. Time-resolved analysis and visualization of dynamic processes in living cells. Proc. Natl. Acad. Sci. USA 96, 7950-7955.

Mintz, P., S.D. Patterson, A.F. Neuwald, C.S. Spahr, and D.L. Spector. 1999. Purification and biochemical characterization of interchromatin granule clusters. EMBO J. 18, 4308-4320.

Lorenz, P., B.F. Baker, C.F. Bennett, and D.L. Spector. 2000. Nucleocytoplasmic shuttling: A novel in vivo property of antisense phosphorothioate oligodeoxynucleotides. Nucleic Acids Research 28, 582-592.

Eils, R., D. Gerlich, W. Tvaruskó, D.L. Spector, and T. Misteli. 2000. Quantitative imaging of pre-mRNA splicing factors in living cells. Molec. Biol. Cell 11, 413-418.

Mintz, P.J. and D.L. Spector. 2000. Compartmentalization of RNA processing factors within nuclear speckles. J. Struct. Biol. 129, 141-151.

Tsukamoto, T., N. Hashiguchi, S.M. Janicki, T. Tumbar, A.S. Belmont and D.L. Spector. 2000. Visualization of gene activity in living cells. Nature Cell Biology 2, 871-878.

Heard, E., C. Rougeulle, D. Arnaud, P. Avner, C.D. Allis and D.L. Spector. 2001. Methylation of histone H3 at Lys-9 is an early mark on the X chromosome during X-inactivation. Cell 107, 727-738.

Spector, D.L. 2001. Nuclear domains. J. Cell Science 114, 2891-2893.

Boggs, B.A., P. Cheung, E. Heard, D.L. Spector, A.C. Chinault and C.D. Allis. 2002. Differentially methylated forms of histone H3 show unique association patterns with inactive human X chromosomes. Nature Genetics 30, 73-76. 

Muratani, M., D. Gerlich, S.M. Janicki, M. Gebhard, R. Eils and D.L. Spector. 2002. Metabolic energy-dependent movement of PML bodies within the mammalian cell nucleus. Nature Cell Biology, 4, 106-110.

Sacco-Bubulya, P. and D.L. Spector. 2002. Disassembly of interchromatin granule clusters alters the coordination of transcription and pre-mRNA splicing. J. Cell Biol. 156, 425-436.

Prasanth, K.V., Sacco-Bubulya, P.A., Prasanth, S.G. and D. L. Spector. 2003. Sequential entry of components of the gene expression machinery into daughter nuclei. Molec. Biol. Cell. 14, 1043-1057.

Janicki, S.M. and D.L. Spector. 2003. Nuclear choreography: interpretations from living cells. Curr. Opinion in Cell Biol. 15, 149-157.

Lamond, A.I. and D.L. Spector. 2003. Nuclear speckles: a model for nuclear organelles. Nature Reviews: Molec. and Cell. Biol. 4: 605-612.

Spector, D.L. 2003. The dynamics of chromosome organization and gene regulation. Ann. Review Biochem. 72: 573-608.

Janicki, S.M., Tsukamoto, T., Salghetti, S.E., Tansey, W.P., Sachidanandam, R., Prasanth, K.V., Ried, T., Shav-Tal, Y., Bertrand, E., Singer, R.H. and Spector, D.L. 2004. From silencing to gene expression: Real-time analysis in single cells. Cell 116, 683-698.

Fang, Y. and Spector, D.L. 2004. The organization and dynamics of SR splicing factors in Arabidopsis thaliana. Molec. Biol. Cell. 15, 2664-2673.

Shav-Tal, Y., Darzacq, X., Shenoy, S.M., Fusco, D., Janicki, S.M., Spector, D.L. and R.H. Singer. 2004. Dynamics of single mRNPs in nuclei of living cells. Science 304, 1797-1800.

Ono, T., Fang, Y., Spector, D.L. and Hirano, T. 2004. Condensins I and II contribute to the structural and functional organization of the centromere/kinetochore region in human cells. Molec. Biol. Cell. 15, 3296-330.

Prasanth, S.G., Prasanth, K.V., Siddiqui, K., Spector, D.L. and Stillman, B. 2004. Human Orc2 localizes to centrosomes, centromeres and heterochromatin during chromosomal inheritance. EMBO J. 23, 2651-2663.

Saitoh, N., Spahr, C.S., Patterson, S., Sacco-Bubulya, P., Newald, A.F. and Spector, D.L. 2004. Proteomic analysis of interchromatin granule clusters. Molec. Biol. Cell 15, 3876-3890.

Bubulya, P.A. and D.L. Spector. 2004. On the "move"ments of nuclear components in living cells. Exp. Cell Res. 296, 4-11.

Bubulya, P.A., Prasanth, K.V., Deerinck, T.J., Gerlich, D., Beaudouin, J., Ellisman, M.H., Ellenberg, J. and Spector, D.L.  2004.  Hypophosphorylated SR splicing factors transiently localize around active nucleolar organizing regions in telophase daughter nuclei.  J. Cell Biol. 167, 51-63.

Fang, Y. and Spector, D.L. 2005. Centromere positioning and dynamics in living Arabidopsis plants. Molec. Biol. Cell 16, 5710-5718.

Prasanth, K.V., Prasanth, S.G., Xuan, Z., Hearn, S., Freier, S.M., Bennett, C.F., Zhang, M.Q. and Spector, D.L. 2005. Regulating gene expression through RNA nuclear retention. Cell 123, 249-263.

May, B.P., Lippman, Z.B., Fang, Y., Spector, D.L. and Martienssen, R.A. 2005. Differential regulation of strand-specific transcripts from Arabidopsis centomeric satellite repeats. PLoS Genetics 1, 705-714.

Politz, J.C.R., Tuft, R.A., Prasanth, K.V., Baudendistel, N., Fogarty, K.E., Lifshitz, L.M., Longowski, J., Spector, D.L. and Pederson, T. 2006. Rapid, diffusional shuttling of poly (A) RNA between nuclear speckles and the nucleoplasm. Mol. Biol. Cell 17, 1239-1249.

Lee. H., Quinn, J.C., Prasanth, K.V., Swiss, V.A., Economides, K.D., Camacho, M.M., Spector, D.L. and Abate-Shen, C. 2006. PIAS1 confers DNA binding specificity on the Msx1 homeoprotein. Genes & Dev. 20, 784-794.

Prasanth, K. V., and D.L. Spector. 2005. The Cell Nucleus. Encyclopedia of Life Sciences. John Wiley and Sons, Inc. doi: 10.1038/npg.eds.0001337 (online).

Spector, D.L. SnapShot: Cellular bodies. 2006. Cell 127, 1071-1072.

Fang, Y. and Spector, D.L. 2007. Identification of nuclear dicing bodies containing proteins for microRNA biogenesis in living Arabidopsis plants. Current Biology 17, 818-823.

Prasanth, K.V. and Spector, D.L. 2007. Eukaryotic regulatory RNAs: an answer to the “genome complexity” conundrum. Genes and Dev. 21, 11-42.

Kumaran, R.I. and Spector, D.L. 2008. A genetic locus targeted to the nuclear periphery in living cells maintains its transcriptional competence. J. Cell Biol. 180, 51-65.

Chen, Y-C. M, Kappel, C., Beaudouin, J., Eils, R. and Spector, D.L. 2008. Live cell dynamics of promyelocytic leukemia nuclear bodies upon enty into and exit from mitosis. Molec. Biol. Cell 19, 3147-3162.

Kumaran, R.I., R. Thakar and D.L. Spector. 2008. Chromatin dynamics and gene positioning. Cell 132, 929-934.

Wilusz, J., Freier, S.M. and Spector, D.L. 2008. 3’ end processing of a long nuclear-retained non-coding RNA yields a small cytoplasmic RNA. Cell 135, 919-932.

Sunwoo,H., Dinger,M.E., Wilusz, J.E., Amaral, P.P., Mattick, J.S. and Spector, D.L. 2009. MEN e/b nuclear retained non-coding RNAs are up-regulated upon muscle differentiation and are essential components of paraspeckles. Genome Research 19, 347-359.

Wilusz, J.E., H. Sunwoo and D.L. Spector. 2009. Long non-coding RNAs: functional surprises from the RNA world. Genes & Development 23, 1494-1504.

Zhao, R., M.S. Bodnar and D.L. Spector. 2009. Nuclear neighborhoods and gene expression. Curr. Opin. Genet and Dev. Biol. 19, 172-179.

Ben-Ari, Y., Brody, Y., Kinor, N., Mor, A., Tsukamoto, T., Spector, D.L., Singer, R.H., and Shav-Tal, Y. 2010. The life of an mRNA in space and time. J. Cell Sci., 123, 1761-1774.

Bernard*, D., Prasanth*, K.V., Tripathi, V., Colasse, S., Nakamura, T., Xuan, Z., Zhang, M.Q., Sedel, F., Jourdren, L., Coulpier, F., Triller, A., Spector, D.L. and Bessis, A. 2010. A long nuclear retained non-coding RNA regulates synaptogenesis by modulating gene expression. EMBO J. 29, 3082-3093.

Prasanth*, K.V., Camiolo*, M., Chan, G., Tripathi, V., Denis, L., Nakamura, T., Hübner, M. and Spector, D.L. 2010. Nuclear organization and dynamics of 7SK RNA in regulating gene expression. Molec. Biol. Cell 21, 4184-4196.

Oda*, H., Huebner*, M.R., Beck, D.B., Vermeulen, M., Hurwitz, J., Spector, D.L., and Reinberg, D. 2010. Regulation of the histone H4 monomethylase PR-Set7 by CRL4Cdt2-mediated PCNA-dependent degradation during DNA damage. Molec. Cell 40, 364-376.

Wilusz, J.E. and D.L. Spector. 2010. An unexpected ending: Non-canonical 3' end processing mechanisms. RNA 16, 259-266.

Huebner, M.R. and D.L. Spector 2010. Chromatin Dynamics. Annu. Rev. Biophys 39, 471-489.

Spector, D.L. and Goldman, R.D. 2010. Constructing and expressing fluorescent protein fusions. Cold Spring Harb Protoc. http://cshprotocols.cshlp.org/cgi/content/full/2010/11/pdb.top87

Spector, D.L., and Goldman, R.D. 2010. Transfection of Mammalian cells with fluorescent protein fusions. Cold Spring Harb Protoc. http://cshprotocols.cshlp.org/cgi/content/full/2010/11/pdb.prot5517

Spector, D.L. and Lamond, A.I. 2010. Nuclear Speckles. Cold Spring Harb Perspect Biol. http://cshperspectives.cshlp.org/content/3/2/a000646.full.pdf+html

Fang, Y. and Spector, D.L. 2010. Live cell imaging of plants. Cold Spring Harb Protoc. http://cshprotocols.cshlp.org/cgi/content/full/2010/2/pdb.top68

Il-Han, K., Chen, Y.-C.M., Spector, D.L., Eils, R. and Rohr, K. 2011. Non-rigid registration of 2D and 3D dynamic cell nuclei images for improved classification of subcellular particle motion. IEEE Trans Image Proc. 20, 1011-1022.

Mao, Y.S., B. Zhang and D.L. Spector. 2011. Biogenesis and function of nuclear bodies. Trends in Genetics 27, 295-306.

Mao, Y.S., Sunwoo, H., Zhang, B., and Spector, D.L. 2011. Direct visualization of the co-transcriptional assembly of a nuclear body by noncoding RNAs. Nature Cell Biol. 13, 95-101.

Shi, L., Wang, J., Hong, F., Spector, D.L. and Fang, Y. 2011. Four amino acids guide the assembly or disassembly of Arabidopsis histone H3.3-containing nucleosomes. Proc. Natl. Acad. Sci. 108, 10574-10578.

Zhao, R., Nakamura, T., Fu, Y., Lazar, Z., and Spector, D.L. 2011. Gene bookmarking accelerates the kinetics of post-mitotic transcriptional re-activation. Nature Cell Biol., 13, 1295-1304.

Zhang, B., Arun, G., Mao, Y.S., Lazar, Z., Hung, G., Bhattacharjee, G., Xiao, X., Booth, C.J., Wu, J., Zhang, C. and Spector, D.L. 2012. The lncRNA Malat1 is dispensable for mouse development but its transcription plays a cis-regulatory role in the adult. Cell Reports 2, 111-123.