Spector, D.L. 1990. Higher order nuclear organization: 3-dimensional distribution of small nuclear ribonucleoprotein particles. Proc. Natl. Acad. Sci. USA 87: 147-151.
Potashkin, J.A., Derby, R.J., and Spector, D.L. 1990. Differential distribution of factors involved in pre-mRNA processing in the yeast cell nucleus. Mol. Cell. Biol. 10: 3524-3534.
Spector, D.L., Fu, X.-D., and Maniatis, T. 1991. Associations between distinct pre-mRNA splicing components and the cell nucleus. EMBO J. 10: 3467-3481.
Huang, S. and Spector, D.L. 1991. Nascent pre-mRNA transcripts are associated with nuclear regions enriched in splicing factors. Genes & Dev., 5: 2288-2302.
Huang, S. and Spector, D.L. 1992. U1 and U2 snRNAs are present in nuclear speckles. Proc. Natl. Acad. Sci. USA 89: 305-308.
O'Keefe, R.T., Henderson, S.C. and Spector, D.L. 1992. Dynamic organization of DNA replication in mammalian cell nuclei; spatially- and temporally-defined replication of chromosome specific a-satellite DNA sequences. J. Cell Biol., 116: 1095-1110.
Huang, S. and Spector, D.L. 1992. Will the real splicing sites please light up! Current Biology, 2: 188-190.
Spector, D.L., Lark, G. and Huang, S. 1992. Differences in snRNP localization between transformed and non-transformed cells. Mol. Biol. Cell, 3: 555-569.
Jiménez-García, L.F. and Spector, D.L. 1993. In vivo evidence that transcription and splicing are coordinated by a recruiting mechanism. Cell 73: 47-59.
Spector, D.L. 1993. Nuclear organization of pre-mRNA processing. Current Opinion in Cell Biology 5: 442-447.
Jiménez-García, L.F., Green, S.R., Mathews, M.B. and Spector, D.L. 1993. Organization of the double-stranded RNA-activated protein kinase DAI and virus-associated VA RNA1 in adenovirus-2 infected HeLa cells. J. Cell Sci. 106: 11-22.
Spector, D.L. 1993. Macromolecular domains within the cell nucleus. Annu. Rev. Cell Biol. 9: 265-315.
Spector, D.L., O'Keefe, R.T., and Jiménez-García, L.F. 1993. Compartmentalization of transcription and pre-mRNA splicing within the mammalian cell nucleus. Cold Spring Harbor Symp. Quant. Biol. 58: 799-805.
O'Keefe, R.T., Mayeda, A., Sadowski, C.L., Krainer, A.R. and Spector, D.L. 1994. Disruption of pre-mRNA splicing in vivo results in reorganization of splicing factors. J. Cell Biol. 124: 249-260.
Huang, S., Deerinck, T.J., Ellisman, M.H. and Spector, D.L. 1994. In vivo analysis of the stability and transport of nuclear poly(A)+ RNA. J. Cell Biol. 126: 877-899.
Deerinck, T.J., Martone, M.E., Lev-Ram, V., Green, D.P.L., Tsien, R.Y., Spector, D.L., Huang, S. and Ellisman, M.H. 1994. Fluorescence photooxidation with eosin: a method for high resolution immunolocalization and in situ hybridization detection for light and electron microscopy. J. Cell Biol. 126: 901-910.
Jiménez-García, L.F., Segura-Valdez, M de L., Ochs, R.L. and Spector, D.L. 1994. Nucleologenesis: U3 snRNA-containing prenucleolar bodies move to sites of active pre-rRNA transcription after mitosis. Molec. Biol Cell. 5: 955-966.
Tani, T., Derby, R.J., Hiraoka, Y and Spector, D.L. 1995. Nucleolar accumulation of poly(A)+ RNA in heat-shocked yeast cells: Implication of nucleolar involvement in mRNA transport. Molec. Biol. Cell 6: 1515-1534.
Huang, S. and Spector, D.L. 1996. Intron-dependent recruitment of pre-mRNA splicing factors to sites of transcription. J. Cell Biol. 133: 719-732.
Huang, S. and Spector, D.L. 1996. The dynamic organization of pre-mRNA splicing factors. J. Cellular Biochem. 62: 191-197.
Misteli, T. and Spector, D.L. 1996. Ser/Thr phosphatase 1 modulates the subnuclear distribution of pre-mRNA splicing factors. Molec. Biol. Cell 7: 1559-1572.
Spector, D.L. 1996. Nuclear organization and gene expression. Exp. Cell Res. 229: 189-197.
Misteli, T. and D.L. Spector. 1997. Protein phosphorylation and the nuclear organization of pre-mRNA splicing. Trends in Cell Biol. 7: 135-138.
Huang, S., T.J. Deerinck, M.H. Ellisman and D.L. Spector. 1997. The dynamic organization of the perinucleolar compartment in the cell nucleus. J. Cell Biol. 137: 965-974.
Misteli, T., J.F. Cáceres and D.L. Spector. 1997. The dynamics of a pre-mRNA splicing factor in living cells. Nature 387: 523-527.
Huang, S., T.J. Deerinck, M.H. Ellisman and D.L. Spector. 1997. The dynamic organization of the perinucleolar compartment in the cell nucleus. J. Cell Biol., 137: 965-974.
Powell, R.D., C.M.R. Halsey, D.L. Spector, S.L. Kaurin, J. McCann and J.F. Hainfeld. 1997. A covalent fluorescent-gold immunoprobe: Simultaneous detection of a pre-mRNA splicing factor by light and electron microscopy. J. Histochem. Cytochem., 45: 947-956.
Huang, S. and Spector, D.L. 1997. Pre-mRNA splicing: Nuclear organization of factors and substrates. In: Eukaryotic Messenger RNA Processing: Frontiers in Molecular Biology. (A.R. Krainer, Ed), pp. 37-67.
Huang, S. , A. Mayeda, A.R. Krainer and D.L. Spector. 1997. RCC1 and nuclear organization. Molec. Biol. Cell., 8: 1143-1157.
Misteli. T. and D.L. Spector. 1997. Applications of the green fluorescent protein in cell biology and biotechnology. Nature Biotechnology 15, 961-964.
Spector, D.L. Genes and transcripts in the interphase nucleus. In: Nuclear Organization, Chromatin Structure and Gene Expression, van Driel, R. and Otte, A.P., eds. Oxford University Press, pp. 169-184.
Cáceres, J.F., T. Misteli, G.R. Screaton, D.L. Spector and A.R. Krainer. 1997. Role of the modular domains of SR proteins in subnuclear localization and alternative splicing specificity. J. Cell Biol. 138: 225-238.
Lorenz, P., B.F. Baker, C.F. Bennett and D.L. Spector. 1998. Phosphorothioate antisense oligonucleotides induce the formation of nuclear bodies. Molec. Biol. Cell., 9, 1007-1023.
Misteli, T. and D.L. Spector. 1998. The cellular organization of gene expression. Curr. Opin. Cell Biol. 10, 323-331.
Misteli, T., J.F. Cáceres, J.Q. Clement, A.R. Krainer, M.F. Wilkinson, and D.L. Spector. 1998. Serine phosphorylation of SR proteins is required for their recruitment to sites of transcription in vivo. J. Cell Biol. 143, 297-307.
Misteli, T. and D.L. Spector. 1999. RNA polymerase II targets pre-mRNA splicing factors to transcription sites in vivo. Molecular Cell 3, 697-705.
Tvaruskó, W., M. Bentele, T. Misteli, R. Rudolf, C. Kaether, D.L. Spector, H.H. Gerdes, and R. Eils. 1999. Time-resolved analysis and visualization of dynamic processes in living cells. Proc. Natl. Acad. Sci. USA 96, 7950-7955.
Mintz, P., S.D. Patterson, A.F. Neuwald, C.S. Spahr, and D.L. Spector. 1999. Purification and biochemical characterization of interchromatin granule clusters. EMBO J. 18, 4308-4320.
Lorenz, P., B.F. Baker, C.F. Bennett, and D.L. Spector. 2000. Nucleocytoplasmic shuttling: A novel in vivo property of antisense phosphorothioate oligodeoxynucleotides. Nucleic Acids Research 28, 582-592.
Eils, R., D. Gerlich, W. Tvaruskó, D.L. Spector, and T. Misteli. 2000. Quantitative imaging of pre-mRNA splicing factors in living cells. Molec. Biol. Cell 11, 413-418.
Mintz, P.J. and D.L. Spector. 2000. Compartmentalization of RNA processing factors within nuclear speckles. J. Struct. Biol. 129, 141-151.
Tsukamoto, T., N. Hashiguchi, S.M. Janicki, T. Tumbar, A.S. Belmont and D.L. Spector. 2000. Visualization of gene activity in living cells. Nature Cell Biology 2, 871-878.
Heard, E., C. Rougeulle, D. Arnaud, P. Avner, C.D. Allis and D.L. Spector. 2001. Methylation of histone H3 at Lys-9 is an early mark on the X chromosome during X-inactivation. Cell 107, 727-738.
Spector, D.L. 2001. Nuclear domains. J. Cell Science 114, 2891-2893.
Boggs, B.A., P. Cheung, E. Heard, D.L. Spector, A.C. Chinault and C.D. Allis. 2002. Differentially methylated forms of histone H3 show unique association patterns with inactive human X chromosomes. Nature Genetics 30, 73-76.
Muratani, M., D. Gerlich, S.M. Janicki, M. Gebhard, R. Eils and D.L. Spector. 2002. Metabolic energy-dependent movement of PML bodies within the mammalian cell nucleus. Nature Cell Biology, 4, 106-110.
Sacco-Bubulya, P. and D.L. Spector. 2002. Disassembly of interchromatin granule clusters alters the coordination of transcription and pre-mRNA splicing. J. Cell Biol. 156, 425-436.
Prasanth, K.V., Sacco-Bubulya, P.A., Prasanth, S.G. and D. L. Spector. 2003. Sequential entry of components of the gene expression machinery into daughter nuclei. Molec. Biol. Cell. 14, 1043-1057.
Janicki, S.M. and D.L. Spector. 2003. Nuclear choreography: interpretations from living cells. Curr. Opinion in Cell Biol. 15, 149-157.
Lamond, A.I. and D.L. Spector. 2003. Nuclear speckles: a model for nuclear organelles. Nature Reviews: Molec. and
Cell. Biol. 4: 605-612.
Spector, D.L. 2003. The dynamics of chromosome organization and gene regulation. Ann. Review Biochem. 72:
Janicki, S.M., Tsukamoto, T., Salghetti, S.E., Tansey, W.P., Sachidanandam, R., Prasanth, K.V., Ried, T., Shav-Tal, Y., Bertrand, E.,
Singer, R.H. and Spector, D.L. 2004. From silencing to gene expression: Real-time analysis in single cells. Cell 116, 683-698.
Fang, Y. and Spector, D.L. 2004. The organization and dynamics of SR splicing factors in Arabidopsis thaliana. Molec. Biol. Cell. 15, 2664-2673.
Shav-Tal, Y., Darzacq, X., Shenoy, S.M., Fusco, D., Janicki, S.M., Spector, D.L. and R.H. Singer. 2004. Dynamics of single mRNPs in nuclei of living cells. Science 304, 1797-1800.
Ono, T., Fang, Y., Spector, D.L. and Hirano, T. 2004. Condensins I and II contribute to the structural and functional organization of the centromere/kinetochore region in human cells.
Molec. Biol. Cell. 15, 3296-330.
Prasanth, S.G., Prasanth, K.V., Siddiqui, K., Spector, D.L. and Stillman, B. 2004. Human Orc2 localizes to centrosomes, centromeres and heterochromatin during chromosomal inheritance.
EMBO J. 23, 2651-2663.
Saitoh, N., Spahr, C.S., Patterson, S., Sacco-Bubulya, P., Newald, A.F. and Spector, D.L. 2004. Proteomic analysis of interchromatin granule clusters. Molec. Biol. Cell 15,
Bubulya, P.A. and D.L. Spector. 2004. On the "move"ments of nuclear components in living cells. Exp. Cell Res. 296, 4-11.
Bubulya, P.A., Prasanth, K.V., Deerinck, T.J., Gerlich, D., Beaudouin, J., Ellisman, M.H., Ellenberg, J. and Spector, D.L. 2004. Hypophosphorylated SR splicing factors transiently localize around active nucleolar organizing regions in telophase daughter nuclei. J. Cell Biol. 167, 51-63.
Fang, Y. and Spector, D.L. 2005. Centromere positioning and dynamics in living Arabidopsis plants. Molec. Biol. Cell 16, 5710-5718.
Prasanth, K.V., Prasanth, S.G., Xuan, Z., Hearn, S., Freier, S.M., Bennett, C.F., Zhang, M.Q. and Spector, D.L. 2005. Regulating gene expression through RNA nuclear retention. Cell 123, 249-263.
May, B.P., Lippman, Z.B., Fang, Y., Spector, D.L. and Martienssen, R.A. 2005. Differential regulation of strand-specific transcripts from Arabidopsis centomeric satellite repeats. PLoS Genetics 1, 705-714.
Politz, J.C.R., Tuft, R.A., Prasanth, K.V., Baudendistel, N., Fogarty, K.E., Lifshitz, L.M., Longowski, J., Spector, D.L. and Pederson, T. 2006. Rapid, diffusional shuttling of poly (A) RNA between nuclear speckles and the nucleoplasm. Mol. Biol. Cell 17, 1239-1249.
Lee. H., Quinn, J.C., Prasanth, K.V., Swiss, V.A., Economides, K.D., Camacho, M.M., Spector, D.L. and Abate-Shen, C. 2006. PIAS1 confers DNA binding specificity on the Msx1 homeoprotein. Genes & Dev. 20, 784-794.
Prasanth, K. V., and D.L. Spector. 2005. The Cell Nucleus. Encyclopedia of Life Sciences. John Wiley and Sons, Inc. doi: 10.1038/npg.eds.0001337 (online).
Spector, D.L. SnapShot: Cellular bodies. 2006. Cell 127, 1071-1072.
Fang, Y. and Spector, D.L. 2007. Identification of nuclear dicing bodies containing proteins for microRNA biogenesis in living Arabidopsis plants. Current Biology 17, 818-823.
Prasanth, K.V. and Spector, D.L. 2007. Eukaryotic regulatory RNAs: an answer to the “genome complexity” conundrum. Genes and Dev. 21, 11-42.
Kumaran, R.I. and Spector, D.L. 2008. A genetic locus targeted to the nuclear periphery in living cells maintains its transcriptional competence. J. Cell Biol. 180, 51-65.
Chen, Y-C. M, Kappel, C., Beaudouin, J., Eils, R. and Spector, D.L. 2008. Live cell dynamics of promyelocytic leukemia nuclear bodies upon enty into and exit from mitosis. Molec. Biol. Cell 19, 3147-3162.
Kumaran, R.I., R. Thakar and D.L. Spector. 2008. Chromatin dynamics and gene positioning. Cell 132, 929-934.
Wilusz, J., Freier, S.M. and Spector, D.L. 2008. 3’ end processing of a
long nuclear-retained non-coding RNA yields a small cytoplasmic RNA. Cell
Sunwoo,H., Dinger,M.E., Wilusz, J.E., Amaral, P.P., Mattick, J.S. and
Spector, D.L. 2009. MEN e/b nuclear retained non-coding RNAs are
up-regulated upon muscle differentiation and are essential components of
paraspeckles. Genome Research 19, 347-359.
Wilusz, J.E., H. Sunwoo and D.L. Spector. 2009. Long non-coding RNAs:
functional surprises from the RNA world. Genes & Development 23, 1494-1504.
Zhao, R., M.S. Bodnar and D.L. Spector. 2009. Nuclear neighborhoods and
gene expression. Curr. Opin. Genet and Dev. Biol. 19, 172-179.
Ben-Ari, Y., Brody, Y., Kinor, N., Mor, A., Tsukamoto, T., Spector, D.L., Singer, R.H., and Shav-Tal, Y. 2010. The life of an mRNA in space and time. J. Cell Sci., 123, 1761-1774.
Bernard*, D., Prasanth*, K.V., Tripathi, V., Colasse, S., Nakamura, T., Xuan, Z., Zhang, M.Q., Sedel, F., Jourdren, L., Coulpier, F., Triller, A., Spector, D.L. and Bessis, A. 2010. A long nuclear retained non-coding RNA regulates synaptogenesis by modulating gene expression. EMBO J. 29, 3082-3093.
Prasanth*, K.V., Camiolo*, M., Chan, G., Tripathi, V., Denis, L., Nakamura, T., Hübner, M. and Spector, D.L. 2010. Nuclear organization and dynamics of 7SK RNA in regulating gene expression. Molec. Biol. Cell 21, 4184-4196.
Oda*, H., Huebner*, M.R., Beck, D.B., Vermeulen, M., Hurwitz, J., Spector, D.L., and Reinberg, D. 2010. Regulation of the histone H4 monomethylase PR-Set7 by CRL4Cdt2-mediated PCNA-dependent degradation during DNA damage. Molec. Cell 40, 364-376.
Wilusz, J.E. and D.L. Spector. 2010. An unexpected ending: Non-canonical 3' end processing mechanisms. RNA 16, 259-266.
Huebner, M.R. and D.L. Spector 2010. Chromatin Dynamics. Annu. Rev. Biophys 39, 471-489.
Spector, D.L. and Goldman, R.D. 2010. Constructing and expressing fluorescent protein fusions. Cold Spring Harb Protoc. http://cshprotocols.cshlp.org/cgi/content/full/2010/11/pdb.top87
Spector, D.L., and Goldman, R.D. 2010. Transfection of Mammalian cells with fluorescent protein fusions. Cold Spring Harb Protoc. http://cshprotocols.cshlp.org/cgi/content/full/2010/11/pdb.prot5517
Spector, D.L. and Lamond, A.I. 2010. Nuclear Speckles. Cold Spring Harb Perspect Biol. http://cshperspectives.cshlp.org/content/3/2/a000646.full.pdf+html
Fang, Y. and Spector, D.L. 2010. Live cell imaging of plants. Cold Spring Harb Protoc. http://cshprotocols.cshlp.org/cgi/content/full/2010/2/pdb.top68
Il-Han, K., Chen, Y.-C.M., Spector, D.L., Eils, R. and Rohr, K. 2011. Non-rigid registration of 2D and 3D dynamic cell nuclei images for improved classification of subcellular particle motion. IEEE Trans Image Proc. 20, 1011-1022.
Mao, Y.S., B. Zhang and D.L. Spector. 2011. Biogenesis and function of nuclear bodies. Trends in Genetics 27, 295-306.
Mao, Y.S., Sunwoo, H., Zhang, B., and Spector, D.L. 2011. Direct visualization of the co-transcriptional assembly of a nuclear body by noncoding RNAs. Nature Cell Biol. 13, 95-101.
Shi, L., Wang, J., Hong, F., Spector, D.L. and Fang, Y. 2011. Four amino acids guide the assembly or disassembly of Arabidopsis histone H3.3-containing nucleosomes. Proc. Natl. Acad. Sci. 108, 10574-10578.
Zhao, R., Nakamura, T., Fu, Y., Lazar, Z., and Spector, D.L. 2011. Gene bookmarking accelerates the kinetics of post-mitotic transcriptional re-activation. Nature Cell Biol., 13, 1295-1304.
Zhang, B., Arun, G., Mao, Y.S., Lazar, Z., Hung, G., Bhattacharjee, G., Xiao, X., Booth, C.J., Wu, J., Zhang, C. and Spector, D.L. 2012. The lncRNA Malat1 is dispensable for mouse development but its transcription plays a cis-regulatory role in the adult. Cell Reports 2, 111-123.